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elisa od values by graphpad prism v8  (GraphPad Software Inc)


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    GraphPad Software Inc elisa od values by graphpad prism v8
    Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated <t>from</t> <t>ELISA</t> OD values by the GraphPad Prism <t>v8.</t> ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Elisa Od Values By Graphpad Prism V8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+od+values+by+graphpad+prism+v8/pmc08893246-120-15-14?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    elisa od values by graphpad prism v8 - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Waning immune responses against SARS-CoV-2 variants of concern among vaccinees in Hong Kong"

    Article Title: Waning immune responses against SARS-CoV-2 variants of concern among vaccinees in Hong Kong

    Journal: EBioMedicine

    doi: 10.1016/j.ebiom.2022.103904

    Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Inhibition, Neutralization, Ex Vivo, Expressing, MANN-WHITNEY



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    Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated <t>from</t> <t>ELISA</t> OD values by the GraphPad Prism <t>v8.</t> ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Elisa Od Values By Graphpad Prism V8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+od+values+by+graphpad+prism+v8/pmc08893246-120-15-14?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    elisa od values by graphpad prism v8 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: EBioMedicine

    Article Title: Waning immune responses against SARS-CoV-2 variants of concern among vaccinees in Hong Kong

    doi: 10.1016/j.ebiom.2022.103904

    Figure Lengend Snippet: Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group.

    Techniques: Enzyme-linked Immunosorbent Assay, Inhibition, Neutralization, Ex Vivo, Expressing, MANN-WHITNEY